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Dongre, C. and van Weerd, J. and Bellini, N. and Osellame, R. and Cerullo, G. and van Weeghel, R. and Hoekstra, H.J.W.M. and Pollnau, M.
(2010)
Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip.
Biomedical Optics Express, 1 (2).
pp. 729-735.
ISSN 2156-7085
Full text available as:
Official URL: http://dx.doi.org/10.1364/BOE.1.000729  AbstractWe present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically.
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