Nederlands
Contact
Search
OrganisationEEMCS EPrints Service
 |
|
|
||||||||||||||||||||||||||||||||||||
|
|
|
|
|
||||||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||||
|
|
|
|
|
||||||||||||||||||||||||||||||||||
|
|
Brivio, M. and Fokkens, R.H. and Verboom, W. and Reinhoudt, D.N. and Tas, N.R. and Goedbloed, M.H. and van den Berg, A.
(2002)
Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry.
Analytical chemistry, 74 (16).
pp. 3972-3976.
ISSN 0003-2700
*** ISI Impact 5,856 ***
Full text available as:
Official URL: http://dx.doi.org/10.1021/ac020185n AbstractA continuous flow micro total analysis system (μ-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the μ-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.
Export this item as: To request a copy of the PDF please email us request copy To correct this item please ask your editor Repository Staff Only: edit this item |
|
|
||||||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||||
|
|
|
|
|
||||||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||||
